Antroquinonol Exerts Immunosuppressive Effect on CD8 + T Cell Proliferation and Activation to Resist Depigmentation Induced by H 2 O 2

Antroquinonol was investigated as antioxidant and inhibition of inflammatory responses. Our study was to evaluate its immunosuppressive effect on CD8 T cells and protective effect on depigmentation. CD8 T cells were treated with antroquinonol , and C57BL/6 mice were treated with antroquinonol with or without H O for 50 consecutive days. We found antroquinonol could inhibit proliferation of CD8 T cells and suppress the production of cytokines IL-2 and IFN- and T cell activation markers CD69 and CD137 . H O treatment induced depigmentation and reduced hair follicle length, skin thickness, and tyrosinase expression . Whereas, antroquinonol obviously ameliorated depigmentation of mice skin and resisted the reduction of hair follicle length, skin thickness, and tyrosinase expression induced by H O . Antroquinonol decreased CD8 T cell infiltration in mice skin, inhibited the production of IL-2 and IFN- , and decreased the expression of CXCL10 and CXCR3. Summarily, our data shows antroquinonol inhibits CD8 T cell proliferation . It also reduces CD8 T cell infiltration and proinflammatory cytokine secretion and suppresses the thinning of epidermal layer . Our findings suggest that antroquinonol exerts immunosuppressive effects on CD8 T cell proliferation and activation to resist depigmentation induced by H O .