An Alternative Approach to Assessing Volume-of-Interest Accuracy Using CBCT and ImageJ Software: In Vitro Study

Aim. This study was performed to evaluate the accuracy and reliability of ImageJ software that we utilized to quantify the volume-of-interest of buccal bone, in vitro study made with the dry skull of a sheep, and the comparison was applied with a reference method. Materials and Methods. A portion of the alveolar bone has been cut, then we built an acrylic model similar to this portion that we shut off, the model was inserted in the place of the piece that we cut, and we have scanned the skull afterwards. Four measurements were made by two examiners using the manual method and ImageJ software whereby DICOM images were extracted from CBCT. The accuracy and reliability were assessed using Student’s test and ICC, when P≤0.05, the statistics are considered significant, and comparison is made employing SPSS and Excel. Results. The repeated measures of volume-of-interest using the manual method and the software method revealed no significant difference. Conclusion. As there is no statistical difference of volume measurements using manual method and ImageJ software, we concluded that the volume measurements with ImageJ software are reliable and accurate.