Differential expression of cold- and diet-specific genes encoding two carp liver Δ9-acyl-CoA desaturase isoforms

Carp respond to cold by the upregulated expression of Δ9-acyl-CoA desaturase. Here we report the cloning and characterization of Cds2, a second Δ9-acyl CoA-desaturase expressed in carp liver. Both Cds1and Cds2 complemented the ole1 mutation in Saccharomyces cerevisiae, permitting the synthesis of Δ9...

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Veröffentlicht in:American journal of physiology. Regulatory, integrative and comparative physiology integrative and comparative physiology, 2003-01, Vol.284 (1), p.R41-R50
Hauptverfasser: Polley, S. D., Tiku, P. E., Trueman, R. T., Caddick, M. X., Morozov, I. Y., Cossins, A. R.
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Sprache:eng
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Zusammenfassung:Carp respond to cold by the upregulated expression of Δ9-acyl-CoA desaturase. Here we report the cloning and characterization of Cds2, a second Δ9-acyl CoA-desaturase expressed in carp liver. Both Cds1and Cds2 complemented the ole1 mutation in Saccharomyces cerevisiae, permitting the synthesis of Δ9-monounsaturates, confirming their identity as Δ9-desaturases. We demonstrate that under a standard feeding regime it is the Cds2, and not Cds1, transcript that is transiently upregulated during the first few days of cooling from 30°C to 10°C, the period when cold-induced membrane restructuring occurs. Cds2 exists as two differentially spliced transcripts, differing by a small segment from the 3′-untranslated region, the ratio of which varies with temperature. Feeding a diet enriched in saturated fats produced a fourfold increase in Cds1 transcript levels, which was blocked by cooling to 15°C. Cds2 transcript levels, however, showed no substantial response to the saturated diet. Thus carp liver uniquely expresses two isoforms of Δ9-acyl CoA desaturase, possibly formed by a recent duplication event, that are differentially regulated by cooling and dietary treatment.
ISSN:0363-6119
1522-1490
DOI:10.1152/ajpregu.00263.2002