Surface activity of a synthetic lung surfactant containing a phospholipase-resistant phosphonolipid analog of dipalmitoyl phosphatidylcholine

Departments of 1 Pediatrics, 5 Environmental Medicine, and 6 Biomedical Engineering, University of Rochester, Rochester 14642; Departments of 3 Pediatrics and 4 Obstetrics and Gynecology, State University of New York at Buffalo, Buffalo, New York 14214; and 2 Department of Chemistry and Biochemistry, University of Guelph, Guelph, Ontario, Canada N1G 2W1 Submitted 16 October 2002 ; accepted in final form 23 April 2003 Surface activity and sensitivity to inhibition from phospholipase A 2 (PLA 2 ), lysophosphatidylcholine (LPC), and serum albumin were studied for a synthetic C16:0 diether phosphonolipid (DEPN-8) combined with 1.5% by weight of mixed hydrophobic surfactant proteins (SP)-B/C purified from calf lung surfactant extract (CLSE). Pure DEPN-8 had better adsorption and film respreading than the major lung surfactant phospholipid dipalmitoyl phosphatidylcholine and reached minimum surface tensions