Chlamydia pneumoniae in foci of "early" calcification of the tunica media in arteriosclerotic arteries: an incidental presence?

1 Surgical Professorial Unit and 2 Division of Anatomical Pathology, St. Vincent's Hospital, and 3 School of Medical Sciences, University of New South Wales, Sydney, New South Wales, Australia Submitted 7 October 2005 ; accepted in final form 30 October 2005 Only a few previous works investigated the involvement of Chlamydia pneumoniae ( Chlamydophila pneumoniae ) in arterial calcification. The present study investigated a possible association between C. pneumoniae and medial calcification. Carotid artery segments obtained by endarterectomy from 60 patients were examined by PCR and immunohistochemistry to identify the presence of C. pneumoniae . Arterial specimens showing double-positive ( n = 17), double-negative ( n = 22), and single-positive results ( n = 21) were further analyzed by a combination of histology, immunohistochemistry, and electron microscopy. Medial calcification occurred in 10 of 17 (58.8%) C. pneumoniae double-positive arterial specimens, but no medial calcification was observed in any of 22 C. pneumoniae double-negative arterial specimens. Electron microscopy indicated C. pneumoniae in smooth muscle cells (SMCs) in foci of medial calcification. Medial SMCs showing damage to the cytoplasm and basement membrane contained the structures with the appearance of elementary, reticulate, and aberrant bodies of C. pneumoniae . The presence of C. pneumoniae in SMCs was confirmed by electron-microscopic immunocytochemistry. In the extracellular matrix, calcification was observed in C. pneumoniae aberrant bodies that exited the SMCs. The findings offer a new hypothesis of arterial calcification: they suggest that C. pneumoniae infection of medial SMCs may be associated with the pathophysiological events of arteriosclerotic calcification of the tunica media. arteriosclerosis; smooth muscle cells Address for reprint requests and other correspondence: Y. V. Bobryshev, Surgical Professorial Unit, St. Vincent's Hospital Sydney, 234 Victoria St., Darlinghurst NSW 2010, Australia (e-mail: y.bobryshev{at}unsw.edu.au )