Macrophage-dependent impairment of α 2 -adrenergic autoreceptor inhibition of Ca 2+ channels in sympathetic neurons from DOCA-salt but not high-fat diet-induced hypertensive rats

DOCA-salt and obesity-related hypertension are associated with inflammation and sympathetic nervous system hyperactivity. Prejunctional α -adrenergic receptors (α ARs) provide negative feedback to norepinephrine release from sympathetic nerves through inhibition of N-type Ca channels. Increased neuronal norepinephrine release in DOCA-salt and obesity-related hypertension occurs through impaired α AR signaling; however, the mechanisms involved are unclear. Mesenteric arteries are resistance arteries that receive sympathetic innervation from the superior mesenteric and celiac ganglia (SMCG). We tested the hypothesis that macrophages impair α AR-mediated inhibition of Ca channels in SMCG neurons from DOCA-salt and high-fat diet (HFD)-induced hypertensive rats. Whole cell patch-clamp methods were used to record Ca currents from SMCG neurons maintained in primary culture. We found that DOCA-salt, but not HFD-induced, hypertension caused macrophage accumulation in mesenteric arteries, increased SMCG mRNA levels of monocyte chemoattractant protein-1 and tumor necrosis factor-α, and impaired α AR-mediated inhibition of Ca currents in SMCG neurons. α AR dysfunction did not involve changes in α AR expression, desensitization, or downstream signaling factors. Oxidative stress impaired α AR-mediated inhibition of Ca currents in SMCG neurons and resulted in receptor internalization in human embryonic kidney-293T cells. Systemic clodronate-induced macrophage depletion preserved α AR function and lowered blood pressure in DOCA-salt rats. HFD caused hypertension without obesity in Sprague-Dawley rats and hypertension with obesity in Dahl salt-sensitive rats. HFD-induced hypertension was not associated with inflammation in SMCG and mesenteric arteries or α AR dysfunction in SMCG neurons. These results suggest that macrophage-mediated α AR dysfunction in the mesenteric circulation may only be relevant to mineralocorticoid-salt excess. NEW & NOTEWORTHY Here, we identify a contribution of macrophages to hypertension development through impaired α -adrenergic receptor (α AR)-mediated inhibition of sympathetic nerve terminal Ca channels in DOCA-salt hypertensive rats. Impaired α AR function may involve oxidative stress-induced receptor internalization. α AR dysfunction may be unique to mineralocorticoid-salt excess, as it does not occur in obesity-related hypertension.