Parathyroid responsiveness to hypocalcemic and hypercalcemic stimuli in adult growth hormone deficiency after growth hormone replacement

1 Diabetes & Endocrinology, Royal Liverpool University Hospital, Liverpool L7 8XP; and 2 Clinical Chemistry, Royal Liverpool University Hospital, Liverpool L69 3GA, United Kingdom Submitted 11 July 2003 ; accepted in final form 11 January 2004 Adult growth hormone deficiency (AGHD) is associated with osteoporosis. Previous reports have suggested that alterations in parathyroid gland responsiveness to changes in calcium concentration may play a role in the genesis of osteoporosis in untreated AGHD patients. We investigated the endogenous parathyroid hormone [PTH-(1–84)] response to hypocalcemic and hypercalcemic stimuli induced by sodium EDTA and calcium gluconate infusion, respectively, and to PTH-(1–34) infusion in AGHD patients before and during GH replacement (GHR). We have demonstrated that the maximum PTH-(1–84) stimulation and suppression occurred at significantly higher calcium concentrations and in response to smaller changes in calcium concentrations after GHR. The calcemic response to the effects of PTH-(1–34) infusion significantly increased after GHR. The calcium set point (the calcium concentration at which the rate of PTH secretion is one-half of its maximal value) significantly increased in all groups after 3 mo on GHR, and it increased further at 12 mo. Our results suggest increased parathyroid gland sensitivity to smaller changes in serum calcium and increased end-organ sensitivity to the effects of PTH in AGHD patients after GHR. These findings may help us to understand the mechanisms underlying the genesis of osteoporosis in AGHD patients. sodium ethylenediaminetetraacetic acid; calcium gluconate; parathyroid hormone-(1–84); parathyroid hormone-(1–34); calcium set point Address for reprint requests and other correspondence: A. M. Ahmad, Link 7-C, Dept. of Diabetes & Endocrinology, Royal Liverpool Univ. Hospital, Prescot St., Liverpool L7 8XP, UK (E-mail: DRAAHMAD{at}Yahoo.com ).