The Monitoring of Open Circuit Potential of Platinum Electrode in Blood Plasma in Resuscitative Patients

Complications of express diagnosis in patients with different diseases are important problem of clinical medicine. Patients in critical conditions requiring emergency medical care are exposed to special risks. Severe combined trauma is one of the main causes of mortality in intensive care units. Treatment of these pathological states is accompanied by the development of oxidative stress, which indicates an unfavorable prognosis, since oxidative stress often leads to multiple organ dysfunction [1]. In addition, patients in this group have a high risk of developing purulent-septic complications. Thus, assessing the condition of patients with multiple and combined trauma as well as predicting the development of complications in patients in the early period is a very important task. Previously for this purpose open-circuit potential of platinum electrode (OCP), measured in blood plasma or serum, was successfully used for transplant patients. The effectiveness of using this electrochemical parameter in living biological media is based on the fact that it reflects the state of equilibrium in the antioxidant/pro-oxidant system. Analysis of monitoring the values of OCP in the treatment of patients with grafted organs led to the development of criteria for assessing the severity of the patient's condition and predicting the effectiveness of treatment [2]. In the present work 28 patients with severe combined trauma were investigated. OCP measurements in blood plasma samples were carried out according [2] on a platinum electrode against saturated Ag/AgCl electrode. The potentiostat IPC-Compact ("Kronas", Russia) was used. The obtained data showed that the changes in the OCP values of the Pt electrode measured in the blood plasma in above patients had significant differences with respect to values obtained earlier in patients with grafted organs [3]. Unusually high values of potential shifts of the platinum electrode in the blood plasma (ΔOCP) were observed during 1 day. The absolute values of OCP shifts were significantly higher compared with data obtained earlier from patients with grafted organs. For example, the value of the OCP in blood plasma in patient B. shifted on 39 mV to the positive potential region for the first day and for 7 days in the early period on 86 mV (Fig. 1a). At the same time it was obtained that dependence potential on time contained wavelike sections (Fig. 2b). According to [3], like data indicates to inflammatory processes occurring in organ