Fabrication of Disposable Electrodes Based on Green Synthesized Iron Oxide Nanoparticles for Enumeration of Bacteria in Water

Poor drinking water quality is a leading cause of preventable deaths in young children. Identifying contaminated water is a major challenge, especially where water resources are very limited. There is an urgent need to fabricate smart disposable electrodes to detect specific Escherichia coli ATCC- 25922 in water to protect against diseases such as gastroenteritis and acute diarrhea. In this article, we presented a simple method to fabricate disposable electrodes using single-pot green iron oxide nanoparticles. The nanostructure and detailed morphology of the green synthesized iron oxide nanoparticles (Ne-Ne-Fe 3 O 4 -NPs) were analyzed using analytical techniques. Modified Ne-Fe 3 O 4 -NPs developed the disposable electrode and cyclic voltammetry (CV) was used to detect the target bacteria. Under optimized experimental conditions, the signals increased linearly while the concentration range increased from 10 1 −10 7 CFU ml −1 , having a short response of time 35 min. In addition, the fabricated disposable electrode showed dynamic detection range, stability of the electrode, and selectivity and provides a new strategy by using green nanoparticles to detect selected bacteria from water.