Aptamer Functionalized Hydrogel Nanocomposite for Electrochemical Sensing of Progesterone

Among the numerous analytical techniques, electrochemical devices have emerged as a cost-effective tool for point-of-care (POC) diagnosis. Electrochemical biosensing devices combine the peculiar features of the bioreceptors, such as selectivity and specificity, with a cost-effective and miniaturised electronic readout system. In this work, aptamer functionalised AuNCs integrated with a hybrid hydrogel employed as a reliable aptamer modified sensing platform for detecting progesterone (p4). The binding features of the p4 sensor are studied using square wave voltammetry. The p4 sensor was responsive to p4 concentration ranging from 1 to 100 nM with a detection limit of 0.3 ng/mL.