Au Nanoparticle-based Detection of Human Chorionic Gonadotropin Using Electrochemical Impedance Spectroscopy

A disposable screen-printed carbon electrode (SPCE) for point-of-care detection platform was developed for human chorionic gonadotropin (hCG). A sandwich assay employing gold-nanoparticles was characterized with differential pulse voltammetry (DPV) and electrochemical impedance spectroscopy (EIS). hCG was analyzed between 100 pg/mL and 1 ng/mL, and a detection limit of 100 pg/mL was observed with 2-µL sample volume. This versatile platform can be easily adapted for decentralized electrochemical immunosensing of clinically important hormones.