High Sensitivity and Large Dynamic Range Surface Plasmon Resonance Sensing for DNA Hybridization Using Au-Nanoparticle-Attached Probe DNA
In this study, we demonstrate the use of surface plasmon interaction between Au nanoparticles (GPs) and a Au thin film for enhancement of surface plasmon resonance (SPR) spectroscopy sensing for DNA hybridization. In situ DNA hybridization with a Au-nanoparticle-attached probe DNA (DNA-GP) monolayer...
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| Veröffentlicht in: | Japanese Journal of Applied Physics 2005-01, Vol.44 (12L), p.L1544 |
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| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Online-Zugang: | Volltext |
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| Zusammenfassung: | In this study, we demonstrate the use of surface plasmon interaction between Au nanoparticles (GPs) and a Au thin film for enhancement of surface plasmon resonance (SPR) spectroscopy sensing for DNA hybridization.
In situ
DNA hybridization with a Au-nanoparticle-attached probe DNA (DNA-GP) monolayer is monitored by SPR spectroscopy, and the results reveal that the DNA-GP allows detection of unlabeled target DNA with the large dynamic range of 1 pM to 10 µM. |
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| ISSN: | 0021-4922 1347-4065 |
| DOI: | 10.1143/JJAP.44.L1544 |