335 : Ovarian Cortex with Higher Vascularization Density is Associated with Higher PTEN Expression After Transplantation on CAM

Background and Aim: Before being applied on the clinical settings, the establishment of ovarian tissue cryopreservation and transplantation process should be optimum. The vascularization after transplant is depended on the ovarian tissue size, which influence the follicular recruitment. It may then affect the post-transplantation survival. Methods: On chorionic-allantoic membrane (CAM) of 8-days-old, fertilized eggs, goat ovarian tissue samples (Capra hircus) were split into pre- and post-transplantation groups. Each group was subsequently divided into 2.5×2.5×1, 5×5×1, and 10×10×1 mm size. Vascularization density and vascularization surface area was analysed and correlated with PTEN protein expressions. Results: Pre- and post-transplantation samples were taken from a total of 36 strips of ovarian tissue. The vascularization density was found to be the highest in the 2.5×2.5×1 mm transplantation samples compared to the pre-transplantation samples (9.54±3.2; 15.46±1.85; P