Vegetation management and site preparation effects on 13 C isotopic composition in planted white spruce

Moisture availability is the factor that most commonly influences the discrimination against 13 C fixation (Δ) by C 3 plants. Therefore, by changing the availability of moisture by way of controlling competing vegetation, Δ in white spruce (Picea glauca (Moench) Voss) seedlings should be affected. The objective of this study was to determine the influence of manual brushing on Δ in white spruce seedlings planted in disc-trenched and control (i.e., no site preparation) microsites. The effects of site preparation and vegetation management on soil moisture, photosynthetically active radiation (PAR), and Δ in white spruce seedlings were evaluated over three growing seasons. Vegetation management increased the amount of PAR reaching seedlings in the control and disc-trenched treatments by removing the shading by native vegetation around each seedling. It appears that the increase in PAR reaching seedlings decreased Δ by increasing the photosynthetic consumption of CO 2 . Differences in soil available moisture (up to 22%) between control and disc-trenched treatments were not reflected in Δ values, contrary to our initial hypothesis. This may indicate that the site was not moisture limiting. Also, these results underline the complexity and difficulty of determining the controlling mechanisms by which Δ is affected.