Chromatographic analysis of methyl ethers of 2-amino-2-deoxy-D-glucopyranose

The mono-, di-, and tri-O-methyl ether derivatives of 2-amino-2-deoxy- D -glucopyranose have been analyzed by gas-liquid chromatography of their fully acetylated 2-acetamido-2-deoxy- D -glucitol derivatives which were prepared from the glycoses by reduction with sodium borohydride followed by acetylation with acetic anhydride. The methyl ethers of 2-amino-2-deoxy- D -glucopyranose were also characterized by degradation with ninhydrin to the corresponding methyl ether derivatives of D -arabinose which were identified by paper chromatography.