A facile methodology for the synthesis and detection of N7-guanine adduct of sulfur mustard as a biomarker

A facile methodology for the synthesis and detection of N7-guanine adduct of sulfur mustard as a biomarker

A simple and convenient method has been developed for the synthesis of a N7-guanine adduct of sulfur mustard (SM), which can serve as an efficient biomarker for alleged exposure to SM. The adduct obtained in high yields (~78%) was characterized on the basis of its physical properties and spectral da...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Canadian journal of chemistry 2002-05, Vol.80 (5), p.504-509
Hauptverfasser: Rao, Mula Kameswara, Bhadury, Pinaki S, Sharma, Mamta, Dangi, Rajinder S, Bhaskar, Appanabhotla S, Raza, Syed K, Jaiswal, Devendra K
Format: Artikel
Sprache:eng
Schlagworte:
Biology
Chemistry
Mustard gas
Physical properties
Sulfur
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:A simple and convenient method has been developed for the synthesis of a N7-guanine adduct of sulfur mustard (SM), which can serve as an efficient biomarker for alleged exposure to SM. The adduct obtained in high yields (~78%) was characterized on the basis of its physical properties and spectral data. In addition, HPLC and LC-MS conditions have been standardized for the detection of the adduct in both in vitro and in vivo samples, which is useful for retrospective detection of exposure to SM in biological samples.Key words: sulfur mustard, biomarker, N7-guanine adduct, in vitro, in vivo, HPLC, LC-MS, electrospray, 2D NMR.
ISSN:0008-4042
1480-3291
DOI:10.1139/v02-058