Evaluation Of Hpv Dna Typing In Sentinel Lymph Node In Patients With Cervical Cancer

The aim of this study was to analyze the HPV DNA types in both sentinel lymph node (SLN) and primary lesion of cervix in patients with cervical cancer. 45 patients who underwent radical hysterectomy and pelvic lymphadenectomy with cervical cancer were enrolled in this prospective study. SLN and primary lesion of the cervix were evaluated with histopathologic examination as well as HPV DNA typing using HPV oligonucleotide microarray. The cases of HPV 16 or 18 single infection were 28 (62.3%) in primary lesion of the cervix and 35(74%) in SLN. Sentinel node detection and frozen biopsy were performed on all 45 subjects. There were 33 benign and 12 malignant cases on frozen biopsy. On permanent histopathological specimen, there were 31 benign and 14 malignant cases. HPV 16 was detected in primary lesion of the cervix up to 100% as a pattern of single or multiple infection and 87% in SLN. There was no case that newly detected HPV DNA types in SLN but negative HPV DNA in primary lesion of cervix. This results imply that SLNs have an essential role for biological metastasis to secondary pelvic lymph node from the primary lesion of cervix.