Enzymes of carbohydrate-phosphorus metabolism in the bone marrow and spleen after sympathectomy. Effects of neuropeptide PRP-1

We studied the effects of proline-1 rich-1 polypeptide (PRP-1) on the activity of enzymes of carbohydrate-phosphorus metabolism in intact rats and rats subjected to pharmacological sympathectomy by 6-hydroxydopamine (6-OHDA, 40 mg/kg) in the homogenates of bone marrow and spleen. We found that, in the bone marrow, suppression of the sympathetic nervous system, inducing insignificant inhibition of alkaline phosphatase (by 15%), does not affect glycogen phosphorylase and substantially increases the activity of acid phosphatase (by 92%). In the spleen, these enzymes are more resistant to sympathectomy. Administration of PRP-1 causes different changes in the activity of the enzymes studied. In the bone marrow, the activity of alkaline and acid phosphatase remained unaltered. In contrast, glycogen phosphorylase was substantially activated by PRP-1; its activity in the spleen increased by 57%. In the bone marrow, PRP-1 increased the activity of this enzyme from zero (intact and symapthectozimed animals) to 1650 U. Our data support Galoyan’s hypothesis on the existence of a neurohumoral axis between the hypothalamus, the location of PRP-1 synthesis, and bone marrow.