Preparation and application of a BODIPY-labeled probe for a real-time polymerase chain reaction
A BODIPY-based (4,4-difluoro-1,3,5,7-tetramethyl-4-bora-3a,4a-diaza-s-indacene, TMB) green fluorescent probe for quantitative real-time polymerase chain reaction (qPCR) was synthesized by azide-alkyne cycloaddition. Comparative studies with the analogous fluorescein-based probe were carried out. We...
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| Veröffentlicht in: | Russian journal of bioorganic chemistry 2015-07, Vol.41 (4), p.451-453 |
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| container_title | Russian journal of bioorganic chemistry |
| container_volume | 41 |
| creator | Ilnitskaya, E. V. Kononevich, Yu. N. Muzafarov, A. M. Rzhevskiy, S. A. Shadrin, I. A. Babaev, E. V. Martynov, V. I. Pakhomov, A. A. |
| description | A BODIPY-based (4,4-difluoro-1,3,5,7-tetramethyl-4-bora-3a,4a-diaza-s-indacene, TMB) green fluorescent probe for quantitative real-time polymerase chain reaction (qPCR) was synthesized by azide-alkyne cycloaddition. Comparative studies with the analogous fluorescein-based probe were carried out. We demonstrated that fluorescent probes with the TMB fluorophore can be used in qPCR experiments. |
| doi_str_mv | 10.1134/S1068162015040068 |
| format | Article |
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V. ; Kononevich, Yu. N. ; Muzafarov, A. M. ; Rzhevskiy, S. A. ; Shadrin, I. A. ; Babaev, E. V. ; Martynov, V. I. ; Pakhomov, A. A.</creator><creatorcontrib>Ilnitskaya, E. V. ; Kononevich, Yu. N. ; Muzafarov, A. M. ; Rzhevskiy, S. A. ; Shadrin, I. A. ; Babaev, E. V. ; Martynov, V. I. ; Pakhomov, A. A.</creatorcontrib><description>A BODIPY-based (4,4-difluoro-1,3,5,7-tetramethyl-4-bora-3a,4a-diaza-s-indacene, TMB) green fluorescent probe for quantitative real-time polymerase chain reaction (qPCR) was synthesized by azide-alkyne cycloaddition. Comparative studies with the analogous fluorescein-based probe were carried out. 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| subjects | Biochemistry Biomedical and Life Sciences Biomedicine Bioorganic Chemistry Letters to the Editor Life Sciences Organic Chemistry |
| title | Preparation and application of a BODIPY-labeled probe for a real-time polymerase chain reaction |
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