Preparation and application of a BODIPY-labeled probe for a real-time polymerase chain reaction

Preparation and application of a BODIPY-labeled probe for a real-time polymerase chain reaction

A BODIPY-based (4,4-difluoro-1,3,5,7-tetramethyl-4-bora-3a,4a-diaza-s-indacene, TMB) green fluorescent probe for quantitative real-time polymerase chain reaction (qPCR) was synthesized by azide-alkyne cycloaddition. Comparative studies with the analogous fluorescein-based probe were carried out. We...

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Veröffentlicht in:Russian journal of bioorganic chemistry 2015-07, Vol.41 (4), p.451-453
Hauptverfasser: Ilnitskaya, E. V., Kononevich, Yu. N., Muzafarov, A. M., Rzhevskiy, S. A., Shadrin, I. A., Babaev, E. V., Martynov, V. I., Pakhomov, A. A.
Format: Artikel
Sprache:eng
Schlagworte:
Biochemistry
Biomedical and Life Sciences
Biomedicine
Bioorganic Chemistry
Letters to the Editor
Life Sciences
Organic Chemistry
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Zusammenfassung:A BODIPY-based (4,4-difluoro-1,3,5,7-tetramethyl-4-bora-3a,4a-diaza-s-indacene, TMB) green fluorescent probe for quantitative real-time polymerase chain reaction (qPCR) was synthesized by azide-alkyne cycloaddition. Comparative studies with the analogous fluorescein-based probe were carried out. We demonstrated that fluorescent probes with the TMB fluorophore can be used in qPCR experiments.
ISSN:1068-1620
1608-330X
DOI:10.1134/S1068162015040068