Effects of amyloid-β peptide Aβ₂₅–₃₅ on glycolytic and antioxidant enzymes in erythrocytes of different ages

Amyloid-β peptide Aβ₂₅–₃₅ was shown to cause lysis of rat erythrocytes of different ages. The toxicity of Aβ₂₅–₃₅ positively correlated with both the erythrocyte age and the peptide concentration. The activity of glycolytic, antioxidant, and Na⁺/K⁺-ATPase enzymes decreased with erythrocyte aging in vivo. In vitro Aβ₂₅–₃₅ reduced the activity of hexokinase, phosphofructokinase, pyruvate kinase, glutathione peroxidase, and glutathione transferase and increased Na⁺/K⁺-ATPase activity in aged erythrocytes to a greater degree than in young cells.