Stability-indicating high performance thin layer chromatographic determination of sulfanilamide in human urine
A simple, sensitive, selective, precise and stability-indicating high-performance thin-layer chromatographic ( HPTLC ) method was developed and applied to human urine for the densitometric determination of sulfanilamide. A mixture of chloroform-ethyl acetate-xylene (2.5: 4.0: 1.0, v/v/v) was used as...
Gespeichert in:
| Veröffentlicht in: | Journal of analytical chemistry (New York, N.Y.) N.Y.), 2009-07, Vol.64 (7), p.714-720 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 720 |
|---|---|
| container_issue | 7 |
| container_start_page | 714 |
| container_title | Journal of analytical chemistry (New York, N.Y.) |
| container_volume | 64 |
| creator | Kilinc, E. Gumgum, B. Hamamci, C. Aydin, F. |
| description | A simple, sensitive, selective, precise and stability-indicating high-performance thin-layer chromatographic (
HPTLC
) method was developed and applied to human urine for the densitometric determination of sulfanilamide. A mixture of chloroform-ethyl acetate-xylene (2.5: 4.0: 1.0, v/v/v) was used as a mobile phase. The system was found to give compact spots for sulfanilamide (retardation factor,
R
f
= 0.21±0.02). The linear regression analysis data for the calibration plots showed good linear relationship with
r
2
= 0.9970 ± 0.0003 and
r
2
= 0.9947 ± 0.020 within the concentration range of 50–250 ng per spot and 100–1000 ng per spot with respect to peak area, respectively. The limit of detection (
LOD
) and quantification (
LOQ
) were 8 and 25 ng per spot, respectively. Sulfanilamide was subjected to acid and alkali hydrolysis, oxidation, dry heat and wet heat treatment. According to the International Conference on Harmonization (
ICH
) guidelines the method was validated for precision, recovery and robustness. The ultraviolet (
UV
) spectra of the degradation products which had different spectra from sulfanilamide were also recorded. |
| doi_str_mv | 10.1134/S1061934809070090 |
| format | Article |
| fullrecord | <record><control><sourceid>crossref_sprin</sourceid><recordid>TN_cdi_crossref_primary_10_1134_S1061934809070090</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>10_1134_S1061934809070090</sourcerecordid><originalsourceid>FETCH-LOGICAL-c288t-4aef1f5c54a04738d0dcb5d05614a4eef64895e8efe325753325e2ae63e855883</originalsourceid><addsrcrecordid>eNp9kM1qwzAQhEVpoWnaB-hNL-BWsiRHPpbQPwj0kPZsNvLKVrDlIMmHvH0V0lshl9mFnW9YhpBHzp44F_J5y1nFayE1q9mKZbkiC14xXQhe19d5z-fidL8ldzHuWbZoXi2I3ybYucGlY-F86wwk5zvau66nBwx2CiN4gzT1ztMBjhio6cM0Qpq6AIfeGdpiwjA6n8nJ08nSOA8WvBtgdC3SzPVzDqFzcB7vyY2FIeLD31ySn7fX7_VHsfl6_1y_bApTap0KCWi5VUZJYHIldMtas1MtUxWXIBFtJXWtUKNFUaqVElmxBKwEaqW0FkvCz7kmTDEGtM0huBHCseGsORXW_CssM-WZidnrOwzNfpqDz29egH4Bywlvww</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Stability-indicating high performance thin layer chromatographic determination of sulfanilamide in human urine</title><source>SpringerNature Journals</source><creator>Kilinc, E. ; Gumgum, B. ; Hamamci, C. ; Aydin, F.</creator><creatorcontrib>Kilinc, E. ; Gumgum, B. ; Hamamci, C. ; Aydin, F.</creatorcontrib><description>A simple, sensitive, selective, precise and stability-indicating high-performance thin-layer chromatographic (
HPTLC
) method was developed and applied to human urine for the densitometric determination of sulfanilamide. A mixture of chloroform-ethyl acetate-xylene (2.5: 4.0: 1.0, v/v/v) was used as a mobile phase. The system was found to give compact spots for sulfanilamide (retardation factor,
R
f
= 0.21±0.02). The linear regression analysis data for the calibration plots showed good linear relationship with
r
2
= 0.9970 ± 0.0003 and
r
2
= 0.9947 ± 0.020 within the concentration range of 50–250 ng per spot and 100–1000 ng per spot with respect to peak area, respectively. The limit of detection (
LOD
) and quantification (
LOQ
) were 8 and 25 ng per spot, respectively. Sulfanilamide was subjected to acid and alkali hydrolysis, oxidation, dry heat and wet heat treatment. According to the International Conference on Harmonization (
ICH
) guidelines the method was validated for precision, recovery and robustness. The ultraviolet (
UV
) spectra of the degradation products which had different spectra from sulfanilamide were also recorded.</description><identifier>ISSN: 1061-9348</identifier><identifier>EISSN: 1608-3199</identifier><identifier>DOI: 10.1134/S1061934809070090</identifier><language>eng</language><publisher>Dordrecht: SP MAIK Nauka/Interperiodica</publisher><subject>Analytical Chemistry ; Chemistry ; Chemistry and Materials Science</subject><ispartof>Journal of analytical chemistry (New York, N.Y.), 2009-07, Vol.64 (7), p.714-720</ispartof><rights>Pleiades Publishing, Ltd. 2009</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c288t-4aef1f5c54a04738d0dcb5d05614a4eef64895e8efe325753325e2ae63e855883</citedby><cites>FETCH-LOGICAL-c288t-4aef1f5c54a04738d0dcb5d05614a4eef64895e8efe325753325e2ae63e855883</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1134/S1061934809070090$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1134/S1061934809070090$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids></links><search><creatorcontrib>Kilinc, E.</creatorcontrib><creatorcontrib>Gumgum, B.</creatorcontrib><creatorcontrib>Hamamci, C.</creatorcontrib><creatorcontrib>Aydin, F.</creatorcontrib><title>Stability-indicating high performance thin layer chromatographic determination of sulfanilamide in human urine</title><title>Journal of analytical chemistry (New York, N.Y.)</title><addtitle>J Anal Chem</addtitle><description>A simple, sensitive, selective, precise and stability-indicating high-performance thin-layer chromatographic (
HPTLC
) method was developed and applied to human urine for the densitometric determination of sulfanilamide. A mixture of chloroform-ethyl acetate-xylene (2.5: 4.0: 1.0, v/v/v) was used as a mobile phase. The system was found to give compact spots for sulfanilamide (retardation factor,
R
f
= 0.21±0.02). The linear regression analysis data for the calibration plots showed good linear relationship with
r
2
= 0.9970 ± 0.0003 and
r
2
= 0.9947 ± 0.020 within the concentration range of 50–250 ng per spot and 100–1000 ng per spot with respect to peak area, respectively. The limit of detection (
LOD
) and quantification (
LOQ
) were 8 and 25 ng per spot, respectively. Sulfanilamide was subjected to acid and alkali hydrolysis, oxidation, dry heat and wet heat treatment. According to the International Conference on Harmonization (
ICH
) guidelines the method was validated for precision, recovery and robustness. The ultraviolet (
UV
) spectra of the degradation products which had different spectra from sulfanilamide were also recorded.</description><subject>Analytical Chemistry</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><issn>1061-9348</issn><issn>1608-3199</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><recordid>eNp9kM1qwzAQhEVpoWnaB-hNL-BWsiRHPpbQPwj0kPZsNvLKVrDlIMmHvH0V0lshl9mFnW9YhpBHzp44F_J5y1nFayE1q9mKZbkiC14xXQhe19d5z-fidL8ldzHuWbZoXi2I3ybYucGlY-F86wwk5zvau66nBwx2CiN4gzT1ztMBjhio6cM0Qpq6AIfeGdpiwjA6n8nJ08nSOA8WvBtgdC3SzPVzDqFzcB7vyY2FIeLD31ySn7fX7_VHsfl6_1y_bApTap0KCWi5VUZJYHIldMtas1MtUxWXIBFtJXWtUKNFUaqVElmxBKwEaqW0FkvCz7kmTDEGtM0huBHCseGsORXW_CssM-WZidnrOwzNfpqDz29egH4Bywlvww</recordid><startdate>20090701</startdate><enddate>20090701</enddate><creator>Kilinc, E.</creator><creator>Gumgum, B.</creator><creator>Hamamci, C.</creator><creator>Aydin, F.</creator><general>SP MAIK Nauka/Interperiodica</general><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20090701</creationdate><title>Stability-indicating high performance thin layer chromatographic determination of sulfanilamide in human urine</title><author>Kilinc, E. ; Gumgum, B. ; Hamamci, C. ; Aydin, F.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c288t-4aef1f5c54a04738d0dcb5d05614a4eef64895e8efe325753325e2ae63e855883</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Analytical Chemistry</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kilinc, E.</creatorcontrib><creatorcontrib>Gumgum, B.</creatorcontrib><creatorcontrib>Hamamci, C.</creatorcontrib><creatorcontrib>Aydin, F.</creatorcontrib><collection>CrossRef</collection><jtitle>Journal of analytical chemistry (New York, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kilinc, E.</au><au>Gumgum, B.</au><au>Hamamci, C.</au><au>Aydin, F.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Stability-indicating high performance thin layer chromatographic determination of sulfanilamide in human urine</atitle><jtitle>Journal of analytical chemistry (New York, N.Y.)</jtitle><stitle>J Anal Chem</stitle><date>2009-07-01</date><risdate>2009</risdate><volume>64</volume><issue>7</issue><spage>714</spage><epage>720</epage><pages>714-720</pages><issn>1061-9348</issn><eissn>1608-3199</eissn><abstract>A simple, sensitive, selective, precise and stability-indicating high-performance thin-layer chromatographic (
HPTLC
) method was developed and applied to human urine for the densitometric determination of sulfanilamide. A mixture of chloroform-ethyl acetate-xylene (2.5: 4.0: 1.0, v/v/v) was used as a mobile phase. The system was found to give compact spots for sulfanilamide (retardation factor,
R
f
= 0.21±0.02). The linear regression analysis data for the calibration plots showed good linear relationship with
r
2
= 0.9970 ± 0.0003 and
r
2
= 0.9947 ± 0.020 within the concentration range of 50–250 ng per spot and 100–1000 ng per spot with respect to peak area, respectively. The limit of detection (
LOD
) and quantification (
LOQ
) were 8 and 25 ng per spot, respectively. Sulfanilamide was subjected to acid and alkali hydrolysis, oxidation, dry heat and wet heat treatment. According to the International Conference on Harmonization (
ICH
) guidelines the method was validated for precision, recovery and robustness. The ultraviolet (
UV
) spectra of the degradation products which had different spectra from sulfanilamide were also recorded.</abstract><cop>Dordrecht</cop><pub>SP MAIK Nauka/Interperiodica</pub><doi>10.1134/S1061934809070090</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1061-9348 |
| ispartof | Journal of analytical chemistry (New York, N.Y.), 2009-07, Vol.64 (7), p.714-720 |
| issn | 1061-9348 1608-3199 |
| language | eng |
| recordid | cdi_crossref_primary_10_1134_S1061934809070090 |
| source | SpringerNature Journals |
| subjects | Analytical Chemistry Chemistry Chemistry and Materials Science |
| title | Stability-indicating high performance thin layer chromatographic determination of sulfanilamide in human urine |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-22T03%3A21%3A14IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-crossref_sprin&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Stability-indicating%20high%20performance%20thin%20layer%20chromatographic%20determination%20of%20sulfanilamide%20in%20human%20urine&rft.jtitle=Journal%20of%20analytical%20chemistry%20(New%20York,%20N.Y.)&rft.au=Kilinc,%20E.&rft.date=2009-07-01&rft.volume=64&rft.issue=7&rft.spage=714&rft.epage=720&rft.pages=714-720&rft.issn=1061-9348&rft.eissn=1608-3199&rft_id=info:doi/10.1134/S1061934809070090&rft_dat=%3Ccrossref_sprin%3E10_1134_S1061934809070090%3C/crossref_sprin%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true |