Optimization of the Production Method for Recombinant Chymosin in the Methylotrophic Yeast Komagataella phaffii

An effective recombinant strain of Komagataella phaffii for Bos taurus prochymosin production was obtained. A method for the isolation and purification of recombinant chymosin that includes two-stage purification via ion exchange and hydrophobic chromatography has been developed. A highly purified (...

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Veröffentlicht in:	Applied biochemistry and microbiology 2020-11, Vol.56 (6), p.657-661
Hauptverfasser:	Filkin, S. Y., Chertova, N. V., Vavilova, E. A., Zatsepin, S. S., Eldarov, M. A., Sadykhov, E. G., Fedorov, A. N., Lipkin, A. V.
Format:	Artikel
Sprache:	eng
Schlagworte:	Biochemistry Biomedical and Life Sciences Life Sciences Medical Microbiology Microbiology
Online-Zugang:	Volltext
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