Optimization of the Production Method for Recombinant Chymosin in the Methylotrophic Yeast Komagataella phaffii

Optimization of the Production Method for Recombinant Chymosin in the Methylotrophic Yeast Komagataella phaffii

An effective recombinant strain of Komagataella phaffii for Bos taurus prochymosin production was obtained. A method for the isolation and purification of recombinant chymosin that includes two-stage purification via ion exchange and hydrophobic chromatography has been developed. A highly purified (...

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Veröffentlicht in:Applied biochemistry and microbiology 2020-11, Vol.56 (6), p.657-661
Hauptverfasser: Filkin, S. Y., Chertova, N. V., Vavilova, E. A., Zatsepin, S. S., Eldarov, M. A., Sadykhov, E. G., Fedorov, A. N., Lipkin, A. V.
Format: Artikel
Sprache:eng
Schlagworte:
Biochemistry
Biomedical and Life Sciences
Life Sciences
Medical Microbiology
Microbiology
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Zusammenfassung:An effective recombinant strain of Komagataella phaffii for Bos taurus prochymosin production was obtained. A method for the isolation and purification of recombinant chymosin that includes two-stage purification via ion exchange and hydrophobic chromatography has been developed. A highly purified (~90%) recombinant chymosin at a concentration of 4 mg/mL (1000 IMCU/mL) was obtained with a yield of 62%. The developed method can be used in industry.
ISSN:0003-6838
1608-3024
DOI:10.1134/S0003683820060058