Production of humanized F(ab’)2 fragment of rabies blocking antibodies in Pichia pastoris yeast

The yeast strain Pichia pastoris , a producer of humanized F(ab’) 2 fragments of rabies-blocking antibodies, has been obtained. Human chaperone BiP coexpression caused a twofold increase of the immunoglobulins secretion level. The use of Fos and Jun zippers in the composition of heavy chains facilit...

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Veröffentlicht in:	Applied biochemistry and microbiology 2016-07, Vol.52 (4), p.378-383
Hauptverfasser:	Yagudin, T. A., Klyatchko, E. V., Zatsepin, S. S., Morozkina, E. V., Benevolensky, S. V., Shemchukova, O. B., Pozdnyakova, L. P., Solopova, O. N., Sveshnikov, P. G.
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Schlagworte:	Biochemistry Biomedical and Life Sciences Life Sciences Medical Microbiology Microbiology
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creator	Yagudin, T. A. Klyatchko, E. V. Zatsepin, S. S. Morozkina, E. V. Benevolensky, S. V. Shemchukova, O. B. Pozdnyakova, L. P. Solopova, O. N. Sveshnikov, P. G.
description	The yeast strain Pichia pastoris , a producer of humanized F(ab’) 2 fragments of rabies-blocking antibodies, has been obtained. Human chaperone BiP coexpression caused a twofold increase of the immunoglobulins secretion level. The use of Fos and Jun zippers in the composition of heavy chains facilitated the dimerization of F(ab’) 2 fragments of the shared pool of secreted immunoglobulins up to 75%.
doi_str_mv	10.1134/S0003683816040165
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title	Production of humanized F(ab’)2 fragment of rabies blocking antibodies in Pichia pastoris yeast
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