Production of humanized F(ab’)2 fragment of rabies blocking antibodies in Pichia pastoris yeast

The yeast strain Pichia pastoris , a producer of humanized F(ab’) 2 fragments of rabies-blocking antibodies, has been obtained. Human chaperone BiP coexpression caused a twofold increase of the immunoglobulins secretion level. The use of Fos and Jun zippers in the composition of heavy chains facilitated the dimerization of F(ab’) 2 fragments of the shared pool of secreted immunoglobulins up to 75%.