Cloning of the Penicillium canescens endo-1,4-β-glucanase gene egl3 and the characterization of the recombinant enzyme

The gene egl3 of the filamentous fungus Penicillium canescens endo-1,4-β-glucanase, belonging to family 12 glycosyl hydrolases, was cloned and sequenced. The gene was expressed in P. canescens under the control of the strong promoter of gene bgaS, coding for β-galactosidase of this fungus, and effic...

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Veröffentlicht in:Applied biochemistry and microbiology 2009-03, Vol.45 (2), p.143-149
Hauptverfasser: Chulkin, A. M, Loginov, D. S, Vavilova, E. A, Abyanova, A. R, Zorov, I. N, Kurzeev, S. A, Koroleva, O. V, Benevolensky, S. V
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Sprache:eng
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Zusammenfassung:The gene egl3 of the filamentous fungus Penicillium canescens endo-1,4-β-glucanase, belonging to family 12 glycosyl hydrolases, was cloned and sequenced. The gene was expressed in P. canescens under the control of the strong promoter of gene bgaS, coding for β-galactosidase of this fungus, and efficient endoglucanase producer strains were obtained. The recombinant protein was isolated from the culture liquid of the producer strain EGL3-13 and purified to homogeneity; its specific activity was 31.7 IU; molecular weight, 26 kDa; and pH and temperature optimums, 3.2 and 54°C, respectively. The K m and V m values for CMC hydrolysis were determined; they amounted to 17.1 g/l and 0.31 μM/(mg s), respectively.
ISSN:0003-6838
1608-3024
DOI:10.1134/S0003683809020057