Role of Marek's Disease Virus (MDV)-Encoded U S 3 Serine/Threonine Protein Kinase in Regulating MDV Meq and Cellular CREB Phosphorylation

Marek's disease (MD) is a neoplastic disease of chickens caused by Marek's disease virus (MDV), a member of the subfamily Like other alphaherpesviruses, MDV encodes a serine/threonine protein kinase, U 3. The functions of U 3 have been extensively studied in other alphaherpesviruses; however, the biological functions of MDV U 3 and its substrates have not been studied in detail. In this study, we investigated potential cellular pathways that are regulated by MDV U 3 and identified chicken CREB (chCREB) as a substrate of MDV U 3. We show that wild-type MDV U 3, but not kinase-dead U 3 (U 3-K220A), increases CREB phosphorylation, leading to recruitment of phospho-CREB (pCREB) to the promoter of the CREB-responsive gene and activation of CREB target gene expression. Using U 3 deletion and U 3 kinase-dead recombinant MDV, we identified U 3-responsive MDV genes during infection and found that the majority of U 3-responsive genes were located in the MDV repeat regions. Chromatin immunoprecipitation sequencing (ChIP-seq) studies determined that some U 3-regulated genes colocalized with Meq (an MDV-encoded oncoprotein) recruitment sites. Chromatin immunoprecipitation-PCR (ChIP-PCR) further confirmed Meq binding to the region, which is also regulated by U 3. Furthermore, biochemical studies demonstrated that MDV U 3 interacts with Meq in transfected cells and MDV-infected chicken embryonic fibroblasts in a phosphorylation-dependent manner. Finally, kinase studies revealed that Meq is a U 3 substrate. MDV U 3 thus acts as an upstream kinase of the CREB signaling pathway to regulate the transcription function of the CREB/Meq heterodimer, which targets cellular and viral gene expression. MDV is a potent oncogenic herpesvirus that induces T-cell lymphoma in infected chickens. Marek's disease continues to have a significant economic impact on the poultry industry worldwide. U 3 encoded by alphaherpesviruses is a multifunctional kinase involved in the regulation of various cellular pathways. Using an MDV genome quantitative reverse transcriptase PCR (qRT-PCR) array and chromatin immunoprecipitation, we elucidated the role of MDV U 3 in viral and cellular gene regulation. Our results provide insights into how viral kinase regulates host cell signaling pathways to activate both viral and host gene expression. This is an important step toward understanding host-pathogen interaction through activation of signaling cascades.