Broad Detection of Diverse H5 and H7 Hemagglutinin Genes of Avian Influenza Viruses by Real-Time Reverse Transcription-PCR Using Primer and Probe Sets Containing Mixed Bases

Real-time reverse transcription-PCR (RT-PCR) was developed for broad detection of diverse H5 and H7 genes in Eurasian and American lineages of avian influenza viruses by using primer and probe sets containing mixed bases. Optimal use of the mixed bases enabled us to minimize sequence mismatches and...

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Veröffentlicht in:	Journal of Clinical Microbiology 2010-11, Vol.48 (11), p.4275-4278
Hauptverfasser:	Tsukamoto, Kenji, Noguchi, Daigo, Suzuki, Koutaro, Shishido, Makiko, Ashizawa, Takayoshi, Kim, Min-Chul, Lee, Youn-Jeong, Tada, Tatsuya
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biological and medical sciences Birds DNA Primers - genetics Fundamental and applied biological sciences. Psychology Hemagglutinin Glycoproteins, Influenza Virus - genetics Hemagglutinin Glycoproteins, Influenza Virus - isolation & purification Influenza in Birds - diagnosis Influenza in Birds - virology Microbiology Miscellaneous Molecular Sequence Data Oligonucleotide Probes - genetics Reverse Transcriptase Polymerase Chain Reaction - methods RNA, Viral - genetics Sensitivity and Specificity Sequence Analysis, DNA Virology Virology - methods
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Zusammenfassung:	Real-time reverse transcription-PCR (RT-PCR) was developed for broad detection of diverse H5 and H7 genes in Eurasian and American lineages of avian influenza viruses by using primer and probe sets containing mixed bases. Optimal use of the mixed bases enabled us to minimize sequence mismatches and to broaden the gene detection spectrum without decreasing sensitivity.
ISSN:	0095-1137 1098-660X
DOI:	10.1128/JCM.01264-10