Evaluation of a Caprine Arthritis-Encephalitis Virus/Maedi-Visna Virus Indirect Enzyme-Linked Immunosorbent Assay in the Serological Diagnosis of Ovine Progressive Pneumonia Virus in U.S. Sheep

A caprine arthritis-encephalitis virus (CAEV)/maedi-visna virus (MVV) indirect enzyme-linked immunosorbent assay (iELISA) was validated with samples from U.S. sheep and by the use of radioimmunoprecipitation as the standard for comparison. The sensitivity and the specificity were 86.0% (±5.8%) and 9...

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Veröffentlicht in:	Clinical and Vaccine Immunology 2010-02, Vol.17 (2), p.307-310
Hauptverfasser:	Herrmann-Hoesing, Lynn M, Broughton-Neiswanger, Liam E, Gouine, Kimberly C, White, Stephen N, Mousel, Michele R, Lewis, Gregory S, Marshall, Katherine L, Knowles, Donald P
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antibodies, Viral - blood Arthritis-Encephalitis Virus, Caprine - genetics Arthritis-Encephalitis Virus, Caprine - immunology Arthritis-Encephalitis Virus, Caprine - isolation & purification Caprine arthritis encephalitis virus Cluster Analysis enzyme-linked immunosorbent assay Enzyme-Linked Immunosorbent Assay - methods gag gene indirect enzyme-linked immunosorbent assay Lentivirus Infections - veterinary Lentivirus Infections - virology maedi-visna disease Maedi-visna virus Molecular Sequence Data Ovine progressive pneumonia virus Phylogeny Pneumonia, Progressive Interstitial, of Sheep - diagnosis Pneumonia, Progressive Interstitial, of Sheep - virology Polymorphism, Genetic RNA, Viral - genetics Sensitivity and Specificity Sequence Analysis, DNA serodiagnosis Sheep United States Veterinary Immunology viral antigens Virology - methods Visna maedi virus Visna-maedi virus - genetics Visna-maedi virus - immunology Visna-maedi virus - isolation & purification
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Zusammenfassung:	A caprine arthritis-encephalitis virus (CAEV)/maedi-visna virus (MVV) indirect enzyme-linked immunosorbent assay (iELISA) was validated with samples from U.S. sheep and by the use of radioimmunoprecipitation as the standard for comparison. The sensitivity and the specificity were 86.0% (±5.8%) and 95.9% (±2.9%), respectively. The iELISA format and phylogenetic differences based on the MVV gag sequence contribute to the reduced sensitivity.
ISSN:	1556-679X 1556-6811 1556-679X
DOI:	10.1128/CVI.00349-09