Construction of p16S lux , a Novel Vector for Improved Bioluminescent Labeling of Gram-Negative Bacteria

Construction of p16S lux , a Novel Vector for Improved Bioluminescent Labeling of Gram-Negative Bacteria

A novel vector has been constructed for the constitutive luminescent tagging of gram-negative bacteria by site-specific integration into the 16S locus of the bacterial chromosome. A number of gram-negative pathogens were successfully tagged using this vector, and the system was validated during muri...

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Veröffentlicht in:Applied and environmental microbiology 2007-11, Vol.73 (21), p.7092-7095
Hauptverfasser: Riedel, Christian U., Casey, Pat G., Mulcahy, Heidi, O'Gara, Fergal, Gahan, Cormac G. M., Hill, Colin
Format: Artikel
Sprache:eng
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Zusammenfassung:A novel vector has been constructed for the constitutive luminescent tagging of gram-negative bacteria by site-specific integration into the 16S locus of the bacterial chromosome. A number of gram-negative pathogens were successfully tagged using this vector, and the system was validated during murine infections of living animals.
ISSN:0099-2240
1098-5336
DOI:10.1128/AEM.01394-07