Molecular Survey of the Dissemination of Two bla KPC -Harboring IncFIA Plasmids in New Jersey and New York Hospitals
Klebsiella pneumoniae carbapenemase (KPC)-producing K. pneumoniae strains have spread worldwide and become a major threat in health care facilities. Transmission of bla KPC , the plasmid-borne KPC gene, can be mediated by clonal spread and horizontal transfer. Here, we report the complete nucleotide...
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Veröffentlicht in: | Antimicrobial agents and chemotherapy 2014-04, Vol.58 (4), p.2289-2294 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | Klebsiella pneumoniae
carbapenemase (KPC)-producing
K. pneumoniae
strains have spread worldwide and become a major threat in health care facilities. Transmission of
bla
KPC
, the plasmid-borne KPC gene, can be mediated by clonal spread and horizontal transfer. Here, we report the complete nucleotide sequences of two novel
bla
KPC-3
-harboring IncFIA plasmids, pBK30661 and pBK30683. pBK30661 is 74 kb in length, with a mosaic plasmid structure; it exhibits homologies to several other plasmids but lacks the plasmid transfer operon (
tra
) and the origin of transfer (
oriT
) that are required for plasmid transfer. pBK30683 is a conjugative plasmid with a cointegrated plasmid structure, comprising a 72-kb element that highly resembles pBK30661 (>99.9% nucleotide identities) and an extra 68-kb element that harbors
tra
and
oriT
. A PCR scheme was designed to detect the distribution of
bla
KPC
-harboring IncFIA (pBK30661-like and pBK30683-like) plasmids in a collection of clinical
Enterobacteriaceae
isolates from 10 hospitals in New Jersey and New York. KPC-harboring IncFIA plasmids were found in 20% of 491
K. pneumoniae
isolates, and all carried
bla
KPC-3
. pBK30661-like plasmids were identified mainly in the epidemic sequence type 258 (ST258)
K. pneumoniae
clone, while pBK30683-like plasmids were widely distributed in ST258 and other
K. pneumoniae
sequence types and among non-
K. pneumoniae Enterobacteriaceae
species. This suggests that both clonal spread and horizontal plasmid transfer contributed to the dissemination of
bla
KPC
-harboring IncFIA plasmids in our area. Further studies are needed to understand the distribution of this plasmid group in other health care regions and to decipher the origins of pBK30661-like and pBK30683-like plasmids. |
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ISSN: | 0066-4804 1098-6596 |
DOI: | 10.1128/AAC.02749-13 |