IκB Kinase-β: NF-κB Activation and Complex Formation with IκB Kinase-α and NIK
Activation of the transcription factor nuclear factor kappa B (NF-κB) by inflammatory cytokines requires the successive action of NF-κB-inducing kinase (NIK) and IκB kinase-α (IKK-α). A widely expressed protein kinase was identified that is 52 percent identical to IKK-α. IκB kinase-β (IKK-β) activat...
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Veröffentlicht in: | Science (American Association for the Advancement of Science) 1997-10, Vol.278 (5339), p.866-869 |
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Sprache: | eng |
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Zusammenfassung: | Activation of the transcription factor nuclear factor kappa B (NF-κB) by inflammatory cytokines requires the successive action of NF-κB-inducing kinase (NIK) and IκB kinase-α (IKK-α). A widely expressed protein kinase was identified that is 52 percent identical to IKK-α. IκB kinase-β (IKK-β) activated NF-κB when overexpressed and phosphorylated serine residues 32 and 36 of IκB-α and serines 19 and 23 of IκB-β. The activity of IKK-β was stimulated by tumor necrosis factor and interleukin-1 treatment. IKK-α and IKK-β formed heterodimers that interacted with NIK. Overexpression of a catalytically inactive form of IKK-β blocked cytokine-induced NF-κB activation. Thus, an active IκB kinase complex may require three distinct protein kinases. |
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ISSN: | 0036-8075 1095-9203 |
DOI: | 10.1126/science.278.5339.866 |