Solid-Phase Radioimmunoassay of Protein Biosynthesis

Solid-Phase Radioimmunoassay of Protein Biosynthesis

Solid-phase radioimmunoassay was used for measuring the rate of radioisotope incorporation into a single protein species. Radioactive antigen was measured by its binding to specific antiserum covalently linked to insoluble bromoacetylcellulose. The insoluble antigen-antibody complex was collected on...

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Veröffentlicht in:Science (American Association for the Advancement of Science) 1970-06, Vol.168 (3938), p.1467-1468
Hauptverfasser: Sox, Harold C., Mohit, Behzad
Format: Artikel
Sprache:eng
Schlagworte:
Antigens
Antiserum
Chemical precipitation
Cytoplasm
Globulins
Precipitins
Protein synthesis
Radioimmunoassay
Table salt
Ungulates
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Beschreibung
Zusammenfassung:Solid-phase radioimmunoassay was used for measuring the rate of radioisotope incorporation into a single protein species. Radioactive antigen was measured by its binding to specific antiserum covalently linked to insoluble bromoacetylcellulose. The insoluble antigen-antibody complex was collected on filters for counting. The assay is performed in about 4 hours as compared with several days for methods based on the precipitin reaction.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.168.3938.1467