1-MHz ultrasound stimulates in vitro production of cardiac and cerebrovascular endothelial cell vasodilators

Ultrasound exposure of the heart and brain during vessel occlusion reduces infarct size. Our aim was to study the production of vasodilatory compounds by endothelial cells after ultrasound stimulation. A 1.05-MHz single element transducer was used to insonify primary mouse endothelial cells (ECs) from heart and brain with a 50-cycle tone burst at a pulse repetition frequency of 50 Hz. Two time points were studied after ultrasound exposure: 15 and 45 minutes. In heart ECs, EETs levels increased significantly with 0.5 MPa (139 ± 16%, p