Spherotech‐EDTA combined serum treatment reduces background more effectively as compared to One Lambda Adsorb Out™ and LIFECODES Serum Cleaner in Luminex‐based solid‐phase immunoassays for HLA antibody detection

Spherotech (SPT) microparticles capture non‐specific binding materials present in test serum, and EDTA removes the so called” prozone effect”. This study presents a novel approach of combined SPT‐EDTA serum treatment prior to Luminex HLA antibody testing to remove high background, and prozone effect in a single step process, and compared the efficacy of SPT‐EDTA serum pre‐treatment with AdsorbOut (ADS) and Serum Cleaner (SC) to reduce background in solid phase immunoassays (SPI). A total of 21 serum samples with a history of elevated negative control (NC) values ≥500, and 20 samples with normal NC values were included to assess the potential adverse effects. A problem of high background was noted in 25% of our samples in SPI. We observed 80% effectiveness in reducing NC values