S6 protein kinase activates Juvenile Hormone and vitellogenin production in the cockroach B lattella germanica
Nutritional status regulates different processes, such as growth and development, through TOR (target of rapamycin) and insulin receptor signalling pathways. The ribosomal S6 protein kinase ( S6K ) is a downstream element of both pathways. Using cDNA from the G erman cockroach B lattella germanica (...
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Veröffentlicht in: | Physiological entomology 2017-03, Vol.42 (1), p.10-16 |
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Sprache: | eng |
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Zusammenfassung: | Nutritional status regulates different processes, such as growth and development, through
TOR
(target of rapamycin) and insulin receptor signalling pathways. The ribosomal
S6
protein kinase (
S6K
) is a downstream element of both pathways. Using
cDNA
from the
G
erman cockroach
B
lattella germanica
(
L
.), two
S6K
isoform sequences (
BgS6K
) are cloned. The long isoform differs from the short one by the insertion of a 22‐amino acid duplication, involving the key phosphorylation position
T
hr
390
. As a result of this, the long isoform presents a new, potentially regulatory phosphorylation site.
RNA interference
knockdown of both
BgS6K
isoforms induces an increase in the length of the last nymphal instar, together with a reduction in the
mRNA
levels of a number of enzymes of the Juvenile Hormone biosynthetic pathway in the corpora allata, vitellogenin
mRNA
levels in the fat body and basal oocyte length. Thus,
BgS6K
is important for nymphal development and is necessary for the full induction of Juvenile Hormone synthesis and vitellogenin production in adult females. |
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ISSN: | 0307-6962 1365-3032 |
DOI: | 10.1111/phen.12156 |