Os WS 1 involved in cuticular wax biosynthesis is regulated by osa‐mi R 1848

Cuticular wax forms a hydrophobic layer covering aerial plant organs and acting as a protective barrier against biotic and abiotic stresses. Compared with well‐known wax biosynthetic pathway, molecular regulation of wax biosynthesis is less known. Here, we show that rice O s WS 1 , a member of the m...

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Veröffentlicht in:Plant, cell and environment cell and environment, 2015-12, Vol.38 (12), p.2662-2673
Hauptverfasser: Xia, Kuaifei, Ou, Xiaojin, Gao, Chunzhi, Tang, Huadan, Jia, Yongxia, Deng, Rufang, Xu, Xinlan, Zhang, Mingyong
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Sprache:eng
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Zusammenfassung:Cuticular wax forms a hydrophobic layer covering aerial plant organs and acting as a protective barrier against biotic and abiotic stresses. Compared with well‐known wax biosynthetic pathway, molecular regulation of wax biosynthesis is less known. Here, we show that rice O s WS 1 , a member of the membrane‐bound O ‐acyl transferase gene family, involved in wax biosynthesis and was regulated by an osa‐mi R 1848 . Os WS 1 ‐tagged green fluorescent protein localized to the endoplasmic reticulum (ER). Compared with wild‐type rice, O s WS 1 overexpression plants displayed a 3% increase in total wax, especially a 35% increase in very long‐chain fatty acids, denser wax papillae around the stoma, more cuticular wax crystals formed on leaf and stem surfaces, pollen coats were thicker and more seedlings survived after water‐deficit treatment. In contrast, O s WS 1 ‐RNAi and osa‐mi R 1848 overexpression plants exhibited opposing changes. Gene expression analysis showed that overexpression of osa‐mi R 1848 down‐regulated O s WS 1 transcripts; furthermore, expression profiles of O s WS 1 and osa‐mi R 1848 were inversely correlated in the leaf, panicle and stem, and upon water‐deficit treatment. These results suggest that O s WS 1 is regulated by osa‐mi R 1848 and participates in cuticular wax formation. Does microRNA regulates wax formation in plants? OsWS1, a homolog of membrane‐bound O‐acyltransferase, involved in wax biosynthesis of rice, especially in elongation of very long chain fatty acids, is regulated by osa‐miR1848 in development and in response to drought.
ISSN:0140-7791
1365-3040
DOI:10.1111/pce.12576