R o KSN , a floral repressor, forms protein complexes with R o FD and R o FT to regulate vegetative and reproductive development in rose
FT / TFL 1 family members have been known to be involved in the development and flowering in plants. In rose, R o KSN , a TFL 1 homologue, is a key regulator of flowering, whose absence causes continuous flowering. Our objectives are to functionally validate R o KSN and to explore its mode of action...
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Veröffentlicht in: | The New phytologist 2014-04, Vol.202 (1), p.161-173 |
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Hauptverfasser: | , , , , , , , , , , |
Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | FT
/
TFL
1
family members have been known to be involved in the development and flowering in plants. In rose,
R
o
KSN
, a
TFL
1
homologue, is a key regulator of flowering, whose absence causes continuous flowering. Our objectives are to functionally validate
R
o
KSN
and to explore its mode of action in rose.
We complemented
A
rabidopsis tfl1
mutants and ectopically expressed
R
o
KSN
in a continuous‐flowering (CF) rose. Using different protein interaction techniques, we studied
R
o
KSN
interactions with
R
o
FD
and
R
o
FT
and possible competition.
In
Arabidopsis
,
R
o
KSN
complemented the
tfl1
mutant by rescuing late flowering and indeterminate growth. In CF roses, the ectopic expression of
R
o
KSN
led to the absence of flowering. Different branching patterns were observed and some transgenic plants had an increased number of leaflets per leaf. In these transgenic roses, floral activator transcripts decreased. Furthermore,
R
o
KSN
was able to interact both with
R
o
FD
and the floral activator,
R
o
FT
. Protein interaction experiments revealed that
R
o
KSN
and
R
o
FT
could compete with
R
o
FD
for repression and activation of blooming, respectively.
We conclude that
R
o
KSN
is a floral repressor and is also involved in the vegetative development of rose.
R
o
KSN
forms a complex with
R
o
FD
and could compete with
R
o
FT
for repression of flowering. |
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ISSN: | 0028-646X 1469-8137 |
DOI: | 10.1111/nph.12625 |