ABSCISIC ACID INSENSITIVE 3 regulates abscisic acid‐responsive gene expression with the nuclear factor Y complex through the ACTT ‐core element in P hyscomitrella patens
The phytohormone ABA and the transcription factor ABSCISIC ACID INSENSITIVE 3 ( ABI 3)/ VIVIPAROUS 1 ( VP 1) function in protecting embryos during the desiccation stage of seed development. In a similar signaling pathway, vegetative tissue of the moss P hyscomitrella patens survives desiccation by a...
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Veröffentlicht in: | The New phytologist 2013-07, Vol.199 (1), p.101-109 |
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Sprache: | eng |
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Zusammenfassung: | The phytohormone
ABA
and the transcription factor
ABSCISIC ACID INSENSITIVE
3 (
ABI
3)/
VIVIPAROUS
1 (
VP
1) function in protecting embryos during the desiccation stage of seed development. In a similar signaling pathway, vegetative tissue of the moss
P
hyscomitrella patens
survives desiccation by activating downstream genes (e.g.
LEA
1) in response to
ABA
and
ABI
3.
We show that the
P
p
LEA
1
promoter responds to
P
p
ABI
3 primarily through the
ACTT
‐core element (5′‐
TCC
ACTT
GTC
‐3′), while the
ACGT
‐core
ABA
‐responsive element (
ABRE
) appears to respond to
ABA
alone. We also found by yeast‐two‐hybrid screening that
P
p
ABI
3A interacts with
P
p
NF
‐
YC
1, a subunit of
CCAAT
box binding factor (
CBF
)/nuclear factor
Y
(
NF
‐Y).
P
p
NF
‐
YC
1 increased the activation of the
P
p
LEA
1
promoter when incubated with
P
p
ABI
3
A
, as did
NF
‐
YB
,
NF
‐
YC
, and
ABI
3 from Arabidopsis.
This new response element (
ACTT
) is responsible for activating the
ABI
3‐dependent
ABA
response pathway cooperatively with the nuclear factor
Y
(
NF
‐
Y
) complex. These results further define the regulatory interactions at the transcriptional level for the expression of this network of genes required for drought/desiccation tolerance.
This gene regulatory set is in large part conserved between vegetative tissue of bryophytes and seeds of angiosperms and will shed light on the evolution of this pathway in the green plant lineage. |
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ISSN: | 0028-646X 1469-8137 |
DOI: | 10.1111/nph.12251 |