Transgenic B rassica rapa plants over‐expressing eIF(iso)4E variants show broad‐spectrum Turnip mosaic virus ( TuMV ) resistance

The protein–protein interaction between VPg (viral protein genome‐linked) of potyviruses and eIF4E (eukaryotic initiation factor 4E) or eIF(iso)4E of their host plants is a critical step in determining viral virulence. In this study, we evaluated the approach of engineering broad‐spectrum resistance in C hinese cabbage ( B rassica rapa ) to T urnip mosaic virus ( TuMV ), which is one of the most important potyviruses, by a systematic knowledge‐based approach to interrupt the interaction between TuMV VPg and B . rapa eIF(iso)4E . The seven amino acids in the cap‐binding pocket of eIF(iso)4E were selected on the basis of other previous results and comparison of protein models of cap‐binding pockets, and mutated. Yeast two‐hybrid assay and co‐immunoprecipitation analysis demonstrated that W95L , K150L and W95L/K150E amino acid mutations of B . rapa eIF(iso)4E interrupted its interaction with TuMV VPg . All eIF(iso)4E mutants were able to complement an eIF4E ‐knockout yeast strain, indicating that the mutated eIF(iso)4E proteins retained their function as a translational initiation factor. To determine whether these mutations could confer resistance, eIF(iso)4E W95L , W95L/K150E and eIF(iso)4E wild‐type were over‐expressed in a susceptible C hinese cabbage cultivar. Evaluation of the TuMV resistance of T 1 and T 2 transformants demonstrated that the over‐expression of the eIF(iso)4E mutant forms can confer resistance to multiple TuMV strains. These data demonstrate the utility of knowledge‐based approaches for the engineering of broad‐spectrum resistance in C hinese cabbage.