Effect of nano‐sized, elemental selenium supplement on the proteome of chicken liver

Summary The nano‐sized (100–500 nm) selenium has higher bioavailability and relatively lower toxicity compared to other selenium forms. The objective of the present study was to compare liver proteome profiles of broiler chicken fed with control diet without Se supplementation and diet supplemented with nano‐Se with 4.25 mg/kg DM. Differential proteome analyses were performed by two‐dimensional gel electrophoresis (2D‐PAGE) followed by tryptic digestion and protein identification by liquid chromatography–mass spectrometry (LC‐MS). Seven hundred and eight spots were detected, and 18 protein spots showed significant difference in their intensity (p