Central composite experimental design for ultrasound‐assisted extraction optimization of alkaloid compounds and antioxidant properties from cocoa pod husk (Theobroma cacao L.)

The objective of this research was to identify ultrasound‐assisted extraction (UAE) parameters for maximizing alkaloid yield and antioxidant activity from cocoa pod husk (CPH). The findings indicated that the optimum UAE parameters were extraction temperature 60°C, extraction time 50 min, and solvent to sample ratio 50 ml/g. Under these UAE parameters, total alkaloid content (TAC), alkaloid extraction efficiency (AEE), DPPH radical scavenging capacity (DRSC), and ferric reducing antioxidant power (FRAP) obtained from the CPH were 45.62 mg AE/g dried sample (DS), 63.28%, 16.57, and 2.15 mg TE/g DS, respectively, which were not significantly different (p