Molecular characterization, virulence and horizontal transmission of B eauveria pseudobassiana from D endroctonus micans ( K ug.) ( C oleoptera: C urculionidae)
The great spruce bark beetle, D endroctonus micans ( K ugelann) ( C oleoptera: C urculionidae), has been a potential threat for T urkey and the entire E urasian spruce forests for many years. Control strategies which have been applied so far are still insufficient to prevent its damage. A previous s...
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Veröffentlicht in: | Journal of applied entomology (1986) 2015-06, Vol.139 (5), p.381-389 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | The great spruce bark beetle,
D
endroctonus micans
(
K
ugelann) (
C
oleoptera:
C
urculionidae), has been a potential threat for
T
urkey and the entire
E
urasian spruce forests for many years. Control strategies which have been applied so far are still insufficient to prevent its damage. A previous study has shown that a
B
eauveria
isolate (
ARSEF
9271) proved to be an efficient microbial control agent against the great spruce bark beetle. In this study, this isolate was identified as
B
. pseudobassiana
based on the partial sequence of
EF
1‐
α
and
ITS
sequence. A conidial suspension (1 × 10
8
/ml) of this fungus caused 100% mortality on both larvae and adults of
D
. micans
within 5 and 6 days, respectively. Also, it caused 100% mycosis value on both larvae and adults. Mortality values of horizontal transmission experiments between larvae and adults which were contaminated with 1 × 10
6
/ml spore suspension at 25%, 50%, 75% and 100% rates were determined as 100% after 15 days at 20°C under the laboratory conditions. We also determined the decrease of the damage in spruce wood block (15 × 25 cm) when the contamination rate of the larvae was increased. Our results indicate that
B
. pseudobassiana
ARSEF
9271 seems to be a very promising biocontrol agent against
D
. micans
. |
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ISSN: | 0931-2048 1439-0418 |
DOI: | 10.1111/jen.12181 |