Effect of long-chain triglyceride lipid emulsion on bupivacaine-induced changes in electrophysiological parameters of rabbit Purkinje cells

Lipid emulsions are used in the reversal of local anesthetic toxicity. The aim of this study was to investigate the cellular electrophysiological effects of long‐chain triglyceride lipid emulsion (LCTE) on cardiac action potential characteristics and conduction disturbances induced by bupivacaine. P...

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Veröffentlicht in:Fundamental & clinical pharmacology 2014-10, Vol.28 (5), p.481-488
Hauptverfasser: Lemoine, Sandrine, Rouet, René, Manrique, Alain, Hanouz, Jean-Luc
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Sprache:eng
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Zusammenfassung:Lipid emulsions are used in the reversal of local anesthetic toxicity. The aim of this study was to investigate the cellular electrophysiological effects of long‐chain triglyceride lipid emulsion (LCTE) on cardiac action potential characteristics and conduction disturbances induced by bupivacaine. Purkinje fibers were dissected from the left ventricle of New Zealand white rabbit hearts and superfused with either Tyrode's solution during 30 min (control group), with bupivacaine 10−6 m, 10−5 m, and 5.10−5 m alone, or in the presence of LCTE 0.5%, in addition, LCTE at 0.1%, 0.5%, and 1% was perfused alone. Electrophysiological parameters were recorded using the conventional microelectrode technique (37 °C, 1 Hz frequency). Bupivacaine 5.10−5 m‐induced conduction blocks (8/8 preparations): LCTE 0.5% suppressed the bupivacaine 5.10−5 m‐induced conduction blocks (1/8 preparations). Exposure to bupivacaine 10−6 m, 10−5 m, and 5.10−5 m resulted in a significant decrease in the maximal rate of depolarization (Vmax) (respectively, 25%, 55%, 75%; P 
ISSN:0767-3981
1472-8206
DOI:10.1111/fcp.12058