Genetic variation for egg‐to‐adult survival in D rosophila melanogaster in a set of recombinant inbred lines reared under heat stress in a natural thermal environment
Quantitative trait loci ( QTL ) for thermotolerance were previously identified for adult flies in several mapping populations of D rosophila melanogaster M eigen ( D iptera: D rosophilidae) in the laboratory. However, laboratory assays may not necessarily reflect the performance under heat stress in...
Gespeichert in:
Veröffentlicht in: | Entomologia experimentalis et applicata 2018-10, Vol.166 (10), p.863-872 |
---|---|
Hauptverfasser: | , , , |
Format: | Artikel |
Sprache: | eng |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
Zusammenfassung: | Quantitative trait loci (
QTL
) for thermotolerance were previously identified for adult flies in several mapping populations of
D
rosophila melanogaster
M
eigen (
D
iptera:
D
rosophilidae) in the laboratory. However, laboratory assays may not necessarily reflect the performance under heat stress in the field. For instance, do the heat‐resistance
QTL
regions in the field match the
QTL
for thermotolerance in laboratory studies? To address this and related questions we used a set of recombinant inbred lines (
RIL
), which were originally used to identify
QTL
in the laboratory. We tested egg‐to‐adult survival (
EAS
)
QTL
in a field experiment under naturally varying heat‐stress temperatures in fly cultures reared on a rotting fruit (banana) in summer.
EAS
under heat stress was found to be 3–6× lower (depending on
RIL
) in the field than in the corresponding control at benign temperature (25 °C). Five
QTL
for
EAS
were significant in the field experiment under heat stress, four of them co‐located with plasticity
QTL
, and none of the
QTL
was significant at control temperature. All significant
QTL
overlapped (co‐localized) with thermotolerance
QTL
previously identified in the laboratory. A previously found
QTL
in the middle of chromosome 2 explained near 30% of the phenotypic variance in
EAS
under heat stress in previous studies in the laboratory, but this
QTL
explained only 8% of the
EAS
variation in our field assay. The largest effect on
EAS
was found for an X‐linked
QTL
(cytological range 7B3‐10C3) in the heat‐stress field experiment, explaining a high percentage (14–45%) of the phenotypic variation in
EAS
. The ecological relevance of
QTL
implicated in this study is discussed. |
---|---|
ISSN: | 0013-8703 1570-7458 |
DOI: | 10.1111/eea.12728 |