Wireless and Portable Electrochemical Measurement System Integrated With Screen-Printed Immunosensor for VP28 Protein Detection

In this letter, a wireless portable electrochemical measurement system is designed for the detection of VP28, a critical protein linked to the white spot syndrome virus (WSSV), which causes significant negative impacts on the shrimp farming industry worldwide. The system includes a screen-printed electrochemical immunosensor, an integrated measurement circuit, and a user interface compatible with Windows computers and Android devices. By employing cyclic voltammetry on a gold sensor functionalized with VP28-specific antibodies, the system exhibits a strong linear correlation between percentage variation in peak current and VP28 concentrations ranging from 5 to 25 ng/mL with a coefficient R 2 of 0.994. This system provides a practical solution for onsite detection of VP28 in shrimp farming, with an impressive detection limit of 2.38 ng/mL. It allows for real-time data display on computers or smartphones through both wired and wireless connections, facilitating prompt management of WSSV outbreaks.