Microcystin-LR-induced cytotoxicity and apoptosis in human embryonic kidney and human kidney adenocarcinoma cell lines

Microcystin-LR-induced cytotoxicity and apoptosis in human embryonic kidney and human kidney adenocarcinoma cell lines

Microcystin-LR (MC-LR) is a potent hepatotoxin, and increasing evidence suggests that it might also induce kidney injury. The aim of the present work was to evaluate the cytotoxicity and possible apoptotic effects of MC-LR on a human embryonic kidney cell line (HEK-293) and human kidney adenocarcino...

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Veröffentlicht in:Microbiology (Society for General Microbiology) 2015-04, Vol.161 (Pt 4), p.819-828
Hauptverfasser: Piyathilaka, M A P C, Pathmalal, M M, Tennekoon, K H, De Silva, B G D N K, Samarakoon, S R, Chanthirika, S
Format: Artikel
Sprache:eng
Schlagworte:
Adenocarcinoma - genetics
Adenocarcinoma - metabolism
Apoptosis - drug effects
Caspase 3 - metabolism
Caspase 9 - metabolism
Cell Line, Tumor
Cell Survival - drug effects
Gene Expression Regulation, Neoplastic
HEK293 Cells
Humans
Kidney Neoplasms - genetics
Kidney Neoplasms - metabolism
Microcystins - pharmacology
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Zusammenfassung:Microcystin-LR (MC-LR) is a potent hepatotoxin, and increasing evidence suggests that it might also induce kidney injury. The aim of the present work was to evaluate the cytotoxicity and possible apoptotic effects of MC-LR on a human embryonic kidney cell line (HEK-293) and human kidney adenocarcinoma cell line (ACHN). Cells were exposed for 24 h to pure MC-LR (1.0-200 µM) and the cytotoxic effects were evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and sulphorhodamine B (SRB) cell viability assays. Cell viability in both cell lines was significantly decreased after treatment with MC-LR at 50 µM for 24 h (P
ISSN:1350-0872
1465-2080
DOI:10.1099/mic.0.000046