Nocardioides yefusunii sp. nov., isolated from Equus kiang (Tibetan wild ass) faeces

Two Gram-stain-positive, irregular rod-shaped (0.2-0.5×1.3-2.5 um) strains, HY056 and HY057, were isolated from the faeces of (the largest of the wild asses) collected at different regions from the Qinghai-Tibetan Plateau of PR China. Phylogenetic analyses indicated that strains HY056 and HY057 belong to the genus by sharing a similarity ranging from 96.3 to 97.0 % in the 16S rRNA gene sequence and forming a distinct cluster with JCM 16608 , 78 , Ka25 and XZ17 . The digital DNA-DNA hybridization value of strain HY056 was 96.9 % with strain HY057, but less than 30.0 % with the above four closest relatives. MK-8(H ) was the predominant (91.6 %) respiratory quinone. The cell wall contained ll-2,6-diaminopimelic acid as the diamino acid of the peptidoglycan. The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phospholipid. The predominant fatty acids (>10.0 %) were C ω9 and -C The DNA G+C contents of strains HY056 and HY057 were 68.9 and 69.1 mol%, respectively. -Glucosidase expression was positive, and acid was produced from d-fructose. Strain HY056 (=CGMCC 4.7563 =JCM 33399 ) is assigned as the type strain of a novel species within the genus , for which the name sp. nov is proposed.