Polymorphic Repetitive DNA Sequences in Mycobacterium tuberculosis Detected with a Gene Probe from a Mycobacterium fortuitum Plasmid

Department of Microbiology, University of Surrey, Guildford GU2 5XH, UK ABSTRACT SUMMARY: Clinical isolates of Mycobacterium tuberculosis were shown by Southern blotting to contain DNA sequences hybridizing to a probe derived from a Mycobacterium fortuitum plasmid. Two such M. tuberculosis DNA fragments, isolated from a gene library, were used as probes to show restriction fragment length polymorphism in M. tuberculosis strains by detecting a repetitive sequence apparently located at different points on the chromosome. This could indicate the presence of a transposable element in M. tuberculosis which is partly homologous to a region of the M. fortuitum plasmid. The probes described can be used to fingerprint M. tuberculosis isolates, and in addition are capable of distinguishing M. tuberculosis from Mycobacterium bovis and BCG. Present address: Faculty of Medicine, Universiti Sains Malaysia, 11800 Pinang, Malaysia.